› 1a7a

X-ray diffraction
2.8Å resolution

STRUCTURE OF HUMAN PLACENTAL S-ADENOSYLHOMOCYSTEINE HYDROLASE: DETERMINATION OF A 30 SELENIUM ATOM SUBSTRUCTURE FROM DATA AT A SINGLE WAVELENGTH

Released:
DOI: 10.2210/pdb1a7a/pdb
PDB entry 1a7a coloured by chain, front view.
PDB entry 1a7a coloured by chain, side view.
PDB entry 1a7a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure determination of selenomethionyl S-adenosylhomocysteine hydrolase using data at a single wavelength.
Turner MA, Yuan CS, Borchardt RT, Hershfield MS, Smith GD, Howell PL
Nat Struct Biol 5 369-76 (1998)
PMID: 9586999

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149993 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 432 amino acids
Theoretical weight: 48.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23526 (Residues: 1-432; Coverage: 100%)
Gene names: AHCY, SAHH
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAD 2 x NAD
Ligand ADC 2 x ADC
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C222
Unit cell:
a: 91.93Å b: 168.02Å c: 137.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.247
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Anomalous diffraction in crystallographic phase evaluation.
Hendrickson WA. (2014)
7 more

25 mentions without citation

S-adenosyl-L-homocysteine hydrolase and methylation disorders: yeast as a model system.
Tehlivets et al. (2013)
24 more