› 1aou

Solution NMR

NMR STRUCTURE OF THE FYN SH2 DOMAIN COMPLEXED WITH A PHOSPHOTYROSYL PEPTIDE, 22 STRUCTURES

Released:
DOI: 10.2210/pdb1aou/pdb
PDB entry 1aou coloured by chain, ensemble of 22 models, front view.
PDB entry 1aou coloured by chain, ensemble of 22 models, side view.
PDB entry 1aou coloured by chain, ensemble of 22 models, top view.
Source organisms:
Primary publication:
The SH2 domain from the tyrosine kinase Fyn in complex with a phosphotyrosyl peptide reveals insights into domain stability and binding specificity.
Mulhern TD, Shaw GL, Morton CJ, Day AJ, Campbell ID
Structure 5 1313-23 (1997)
PMID: 9351806

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136550 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase Fyn Chain: F
Molecule details ›
Chain: F
Length: 106 amino acids
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06241 (Residues: 143-248; Coverage: 20%)
  • Best match: P06241-3 (Residues: 143-232)
Gene name: FYN
Sequence domains: SH2 domain
Structure domains: SH2 domain
Middle T antigen Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.47 KDa
Source organism: Mesocricetus auratus polyomavirus 1
UniProt:
  • Canonical: P03079 (Residues: 321-331; Coverage: 3%)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
Refinement method: DYNAMICAL SIMULATED ANNEALING
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

SH2 and PTB domains in tyrosine kinase signaling.
Schlessinger et al. (2003)
1 more

4 mentions without citation

Quantifying information transfer by protein domains: analysis of the Fyn SH2 domain structure.
Lenaerts et al. (2008)
3 more