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DELETION OF A BURIED SALT BRIDGE IN BARNASE

X-ray diffraction
2Å resolution
PDB entry 1b21 coloured by chain, front view.
PDB entry 1b21 coloured by chain, side view.
PDB entry 1b21 coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Ribonuclease
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Multimeric state: monomeric
Accessible surface area: 6167.61 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-132802
Assembly 2
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Assembly Name: Ribonuclease
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Multimeric state: monomeric
Accessible surface area: 6057.94 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-132802
Assembly 3 (preferred)
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Assembly Name: Ribonuclease
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Multimeric state: monomeric
Accessible surface area: 5965.68 Å2
Buried surface area: 72.81 Å2
Dissociation area: 36.4 Å2
Dissociation energy (ΔGdiss): 17.1 kcal/mol
Dissociation entropy (TΔSdiss): -0.22 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-132802

Macromolecules

Ribonuclease
Chains: A, B, C
Length: 110 amino acids
Theoretical weight: 12.33 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 48-157; Coverage: 89%)
Pfam: ribonuclease
InterPro:
CATH: Microbial ribonucleases
SCOP: Bacterial ribonucleases
Ribonuclease in PDB entry 1b21, assembly 3, front view.
Ribonuclease in PDB entry 1b21, assembly 3, side view.
Ribonuclease in PDB entry 1b21, assembly 3, top view.
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