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COMPLEX OF BDELLASTASIN WITH BOVINE TRYPSIN

X-ray diffraction
3.3Å resolution
PDB entry 1c9t coloured by chain, front view.
PDB entry 1c9t coloured by chain, side view.
PDB entry 1c9t coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Bdellastasin and Serine protease 1
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Multimeric state: hetero dimer
Accessible surface area: 11886.36 Å2
Buried surface area: 1241.93 Å2
Dissociation area: 620.97 Å2
Dissociation energy (ΔGdiss): 2.2 kcal/mol
Dissociation entropy (TΔSdiss): 10.37 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-133336
Assembly 2
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Assembly Name: Bdellastasin and Serine protease 1
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Multimeric state: hetero dimer
Accessible surface area: 11793.2 Å2
Buried surface area: 1350.7 Å2
Dissociation area: 675.35 Å2
Dissociation energy (ΔGdiss): 4.79 kcal/mol
Dissociation entropy (TΔSdiss): 10.39 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-133336
Assembly 3
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Assembly Name: Bdellastasin and Serine protease 1
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Multimeric state: hetero dimer
Accessible surface area: 11778.85 Å2
Buried surface area: 1295.27 Å2
Dissociation area: 647.63 Å2
Dissociation energy (ΔGdiss): 4.67 kcal/mol
Dissociation entropy (TΔSdiss): 10.38 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-133336
Assembly 4
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Assembly Name: Bdellastasin and Serine protease 1
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Multimeric state: hetero dimer
Accessible surface area: 11761.15 Å2
Buried surface area: 1286.98 Å2
Dissociation area: 643.49 Å2
Dissociation energy (ΔGdiss): 4.03 kcal/mol
Dissociation entropy (TΔSdiss): 10.37 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-133336
Assembly 5
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Assembly Name: Bdellastasin and Serine protease 1
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Multimeric state: hetero dimer
Accessible surface area: 11913.01 Å2
Buried surface area: 1269.58 Å2
Dissociation area: 634.79 Å2
Dissociation energy (ΔGdiss): 3.22 kcal/mol
Dissociation entropy (TΔSdiss): 10.37 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-133336
Assembly 6
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Assembly Name: Bdellastasin and Serine protease 1
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Multimeric state: hetero dimer
Accessible surface area: 11806.0 Å2
Buried surface area: 1268.11 Å2
Dissociation area: 634.05 Å2
Dissociation energy (ΔGdiss): 3.98 kcal/mol
Dissociation entropy (TΔSdiss): 10.38 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-133336

Macromolecules

Serine protease 1
Chains: A, B, C, D, E, F
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Pfam: Trypsin
InterPro:
CATH: Trypsin-like serine proteases
SCOP: Eukaryotic proteases
Serine protease 1 in PDB entry 1c9t, assembly 1, front view.
Serine protease 1 in PDB entry 1c9t, assembly 1, side view.
Serine protease 1 in PDB entry 1c9t, assembly 1, top view.
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Bdellastasin
Chains: G, H, I, J, K, L
Length: 59 amino acids
Theoretical weight: 6.35 KDa
Source organism: Hirudo medicinalis
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P82107 (Residues: 1-59; Coverage: 100%)
Pfam: Antistasin family
InterPro:
CATH: Antistasin; domain 1
SCOP: Huristasin-like
Bdellastasin in PDB entry 1c9t, assembly 1, front view.
Bdellastasin in PDB entry 1c9t, assembly 1, side view.
Bdellastasin in PDB entry 1c9t, assembly 1, top view.
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