› 1cjt

X-ray diffraction
2.8Å resolution

COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH BETA-L-2',3'-DIDEOXYATP, MN, AND MG

Released:
DOI: 10.2210/pdb1cjt/pdb
PDB entry 1cjt coloured by chain, front view.
PDB entry 1cjt coloured by chain, side view.
PDB entry 1cjt coloured by chain, top view.
Source organisms:
Primary publication:
Two-metal-Ion catalysis in adenylyl cyclase.
Tesmer JJ, Sunahara RK, Johnson RA, Gosselin G, Gilman AG, Sprang SR
Science 285 756-60 (1999)
PMID: 10427002

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-138237 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Adenylate cyclase type 5 Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.5 KDa
Source organism: Canis lupus familiaris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30803 (Residues: 445-661; Coverage: 17%)
Gene name: ADCY5
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase
Adenylate cyclase type 2 Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26769 (Residues: 870-1081; Coverage: 19%)
Gene name: Adcy2
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: C
Molecule details ›
Chain: C
Length: 402 amino acids
Theoretical weight: 46.66 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04896 (Residues: 1-70, 71-394; Coverage: 100%)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand MG 2 x MG
Ligand MN 1 x MN
Ligand FOK 1 x FOK
Ligand DAD 1 x DAD
Ligand MES 1 x MES
Ligand CL 1 x CL
Ligand GSP 1 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P21212
Unit cell:
a: 118.2Å b: 134.2Å c: 71.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

The chemical repertoire of natural ribozymes.
Doudna et al. (2002)
39 more

7 mentions without citation

International Union of Basic and Clinical Pharmacology. CI. Structures and Small Molecule Modulators of Mammalian Adenylyl Cyclases.
Dessauer et al. (2017)
6 more