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INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION

X-ray diffraction
2.1Å resolution
PDB entry 1cz0 coloured by chain, front view.
PDB entry 1cz0 coloured by chain, side view.
PDB entry 1cz0 coloured by chain, top view.
Source organism: Physarum polycephalum
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Intron-encoded endonuclease I-PpoI and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 19372.47 Å2
Buried surface area: 11725.17 Å2
Dissociation area: 4,539.05 Å2
Dissociation energy (ΔGdiss): 84.34 kcal/mol
Dissociation entropy (TΔSdiss): 14.32 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-113567

Macromolecules

Intron-encoded endonuclease I-PpoI
Chains: A, B
Length: 162 amino acids
Theoretical weight: 17.68 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94702 (Residues: 2-163; Coverage: 99%)
Pfam: Zinc-binding loop region of homing endonuclease
InterPro:
CATH: Homing Intron 3 (I-ppo) Encoded Endonuclease; Chain A
SCOP: Intron-encoded homing endonucleases
Intron-encoded endonuclease I-PpoI in PDB entry 1cz0, assembly 1, front view.
Intron-encoded endonuclease I-PpoI in PDB entry 1cz0, assembly 1, side view.
Intron-encoded endonuclease I-PpoI in PDB entry 1cz0, assembly 1, top view.
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DNA chains C, D
Name: DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3')
Representative chains: C, D
Source organism: Physarum polycephalum [5791]
Expression system: Not provided
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3') in PDB entry 1cz0, assembly 1, front view.
DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3') in PDB entry 1cz0, assembly 1, side view.
DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3') in PDB entry 1cz0, assembly 1, top view.
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