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Complex of a chitinase with the natural product cyclopentapeptide argifin from Gliocladium

X-ray diffraction
2Å resolution
PDB entry 1h0i coloured by chain, front view.
PDB entry 1h0i coloured by chain, side view.
PDB entry 1h0i coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Chitinase B and peptide
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Multimeric state: hetero dimer
Accessible surface area: 19857.4 Å2
Buried surface area: 2228.35 Å2
Dissociation area: 502.1 Å2
Dissociation energy (ΔGdiss): -5.14 kcal/mol
Dissociation entropy (TΔSdiss): 6.16 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-145921
Assembly 2
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Assembly Name: Chitinase B and peptide
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Multimeric state: hetero dimer
Accessible surface area: 19967.67 Å2
Buried surface area: 3219.48 Å2
Dissociation area: 548.41 Å2
Dissociation energy (ΔGdiss): 3.1 kcal/mol
Dissociation entropy (TΔSdiss): 6.18 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-145921

Macromolecules

Chitinase B
Chains: A, B
Length: 499 amino acids
Theoretical weight: 55.55 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11797 (Residues: 1-499; Coverage: 100%)
Gene name: chiB
Pfam: Glycosyl hydrolases family 18
InterPro:
CATH:
SCOP:
Chitinase B in PDB entry 1h0i, assembly 1, front view.
Chitinase B in PDB entry 1h0i, assembly 1, side view.
Chitinase B in PDB entry 1h0i, assembly 1, top view.
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ARGIFIN
Chains: C, D
Length: 5 amino acids
Theoretical weight: 694 Da
Source organism: Gliocladium
Expression system: Not provided
ARGIFIN in PDB entry 1h0i, assembly 1, front view.
ARGIFIN in PDB entry 1h0i, assembly 1, side view.
ARGIFIN in PDB entry 1h0i, assembly 1, top view.
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