› 1h1h

X-ray diffraction
2Å resolution

Crystal Structure of Eosinophil Cationic Protein in Complex with 2',5'-ADP at 2.0 A resolution Reveals the Details of the Ribonucleolytic Active site

Released:
DOI: 10.2210/pdb1h1h/pdb
PDB entry 1h1h coloured by chain, front view.
PDB entry 1h1h coloured by chain, side view.
PDB entry 1h1h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of eosinophil cationic protein in complex with 2',5'-ADP at 2.0 A resolution reveals the details of the ribonucleolytic active site.
Mohan CG, Boix E, Evans HR, Nikolovski Z, Nogués MV, Cuchillo CM, Acharya KR
Biochemistry 41 12100-6 (2002)
PMID: 12356310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eosinophil cationic protein Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 15.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12724 (Residues: 28-160; Coverage: 100%)
Gene names: ECP, RNASE3, RNS3
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand A2P 1 x A2P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P63
Unit cell:
a: 100.7Å b: 100.7Å c: 31.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.205 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Nucleotide binding architecture for secreted cytotoxic endoribonucleases.
Boix et al. (2013)
13 other articles

7 mentions without citation

Eosinophil granule proteins: form and function.
Acharya et al. (2014)
6 more