› 1h8a

X-ray diffraction
2.23Å resolution

CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA COMPLEX3

Released:
DOI: 10.2210/pdb1h8a/pdb
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PDB entry 1h8a coloured by chain, front view.
PDB entry 1h8a coloured by chain, side view.
PDB entry 1h8a coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of c-Myb-C/EBP beta cooperation from separated sites on a promoter.
Tahirov TH, Sato K, Ichikawa-Iwata E, Sasaki M, Inoue-Bungo T, Shiina M, Kimura K, Takata S, Fujikawa A, Morii H, Kumasaka T, Yamamoto M, Ishii S, Ogata K
Cell 108 57-70 (2002)
PMID: 11792321

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113887 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
CCAAT/enhancer-binding protein beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 78 amino acids
Theoretical weight: 9.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17676 (Residues: 259-336; Coverage: 23%)
Gene names: CEBPB, PP9092, TCF5
Sequence domains: Basic region leucine zipper
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Transforming protein Myb Chain: C
Molecule details ›
Chain: C
Length: 128 amino acids
Theoretical weight: 15.48 KDa
Source organism: Avian myeloblastosis virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01104 (Residues: 1-122; Coverage: 32%)
Gene name: V-MYB
Sequence domains: Myb-like DNA-binding domain
Structure domains: Homeodomain-like
DNA(5'-(*GP*AP*TP*GP*TP*GP*GP*CP*GP*CP*AP* AP*TP*CP*CP*TP*TP*AP*AP*CP*GP*GP*AP*CP*TP*G)-3') Chain: D
DNA(5'-(*CP*CP*AP*GP*TP*CP*CP*GP*TP*TP*AP* AP*GP*GP*AP*TP*TP*GP*CP*GP*CP*CP*AP*CP*AP*T)-3') Chain: E

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P21212
Unit cell:
a: 109.86Å b: 166.7Å c: 39.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.288
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

CCAAT/enhancer-binding proteins: structure, function and regulation.
Ramji et al. (2002)
10 more

15 mentions without citation

The importance of being flexible: the case of basic region leucine zipper transcriptional regulators.
Miller M. (2009)
14 more

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