› 1i2t

X-ray diffraction
1.04Å resolution

X-RAY STRUCTURE OF THE HUMAN HYPERPLASTIC DISCS PROTEIN: AN ORTHOLOG OF THE C-TERMINAL DOMAIN OF POLY(A)-BINDING PROTEIN

Released:
DOI: 10.2210/pdb1i2t/pdb
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PDB entry 1i2t coloured by chain, front view.
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PDB entry 1i2t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structure of the human hyperplastic discs protein: an ortholog of the C-terminal domain of poly(A)-binding protein.
Deo RC, Sonenberg N, Burley SK
Proc Natl Acad Sci U S A 98 4414-9 (2001)
PMID: 11287654

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131816 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase UBR5 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95071 (Residues: 2393-2453; Coverage: 2%)
Gene names: EDD, EDD1, HYD, KIAA0896, UBR5
Sequence domains: Poly-adenylate binding protein, unique domain
Structure domains: c-terminal domain of poly(a) binding protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 32.969Å b: 38.166Å c: 51.062Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.143 0.171
Expression system: Escherichia coli BL21(DE3)

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Citations

10 review citations

Poly(A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression.
Mangus et al. (2003)
9 more

17 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
16 more

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