› 1jwj

X-ray diffraction
2.6Å resolution

Murine Inducible Nitric Oxide Synthase Oxygenase Dimer (Delta 65) with W457F Mutation at Tetrahydrobiopterin Binding Site

Released:
DOI: 10.2210/pdb1jwj/pdb
PDB entry 1jwj coloured by chain, front view.
PDB entry 1jwj coloured by chain, side view.
PDB entry 1jwj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structures of tetrahydrobiopterin binding-site mutants of inducible nitric oxide synthase oxygenase dimer and implicated roles of Trp457.
Aoyagi M, Arvai AS, Ghosh S, Stuehr DJ, Tainer JA, Getzoff ED
Biochemistry 40 12826-32 (2001)
PMID: 11669619

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-541982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, inducible Chains: A, B
Molecule details ›
Chains: A, B
Length: 433 amino acids
Theoretical weight: 50.08 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29477 (Residues: 66-498; Coverage: 38%)
Gene names: Inosl, Nos2
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
4 bound ligands:
Ligand BOG 2 x BOG
Ligand H4B 2 x H4B
Ligand GOL 9 x GOL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6122
Unit cell:
a: 213.603Å b: 213.603Å c: 116.892Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.218 0.257
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The molecular mechanism of mammalian NO-synthases: a story of electrons and protons.
Santolini J. (2011)
1 more

2 mentions without citation

Use of Computational Biochemistry for Elucidating Molecular Mechanisms of Nitric Oxide Synthase.
Bignon et al. (2019)
1 more