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Murine Inducible Nitric Oxide Synthase Oxygenase Dimer (Delta 65) with W457F Mutation at Tetrahydrobiopterin Binding Site

X-ray diffraction
2.6Å resolution
PDB entry 1jwj coloured by chain, front view.
PDB entry 1jwj coloured by chain, side view.
PDB entry 1jwj coloured by chain, top view.
Source organism: Mus musculus
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Nitric oxide synthase, inducible
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Multimeric state: homo dimer
Accessible surface area: 34202.41 Å2
Buried surface area: 10395.48 Å2
Dissociation area: 2,329.2 Å2
Dissociation energy (ΔGdiss): 27.63 kcal/mol
Dissociation entropy (TΔSdiss): 14.72 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-151523
Assembly 2 (preferred)
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Assembly Name: Nitric oxide synthase, inducible
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Multimeric state: homo dimer
Accessible surface area: 34961.58 Å2
Buried surface area: 11837.67 Å2
Dissociation area: 2,387.31 Å2
Dissociation energy (ΔGdiss): 21.07 kcal/mol
Dissociation entropy (TΔSdiss): 14.74 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-151523

Macromolecules

Nitric oxide synthase, inducible
Chains: A, B
Length: 433 amino acids
Theoretical weight: 50.08 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29477 (Residues: 66-498; Coverage: 38%)
Gene names: Inosl, Nos2
Pfam: Nitric oxide synthase, oxygenase domain
InterPro:
CATH:
SCOP: Nitric oxide (NO) synthase oxygenase domain
Nitric oxide synthase, inducible in PDB entry 1jwj, assembly 2, front view.
Nitric oxide synthase, inducible in PDB entry 1jwj, assembly 2, side view.
Nitric oxide synthase, inducible in PDB entry 1jwj, assembly 2, top view.
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