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Crystal Structure of a Mutant (C109G,G212C) P4-P6 Domain of the Group I Intron from Tetrahymena Thermophilia

X-ray diffraction
2.8Å resolution
PDB entry 1l8v coloured by chain, front view.
PDB entry 1l8v coloured by chain, side view.
PDB entry 1l8v coloured by chain, top view.
Source organism: Synthetic construct
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: RNA
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Multimeric state: monomeric
Accessible surface area: 24951.82 Å2
Buried surface area: 574.29 Å2
Dissociation area: 57.02 Å2
Dissociation energy (ΔGdiss): 10.85 kcal/mol
Dissociation entropy (TΔSdiss): -1.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-196102
Assembly 2
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Assembly Name: RNA
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Multimeric state: monomeric
Accessible surface area: 24944.07 Å2
Buried surface area: 568.36 Å2
Dissociation area: 56.04 Å2
Dissociation energy (ΔGdiss): 10.83 kcal/mol
Dissociation entropy (TΔSdiss): -1.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-196102

Macromolecules

RNA chains A, B
Name: P4-P6 RNA ribozyme domain
Representative chains: A, B
Source organism: Synthetic construct [32630]
Expression system: Not provided
Length: 157 nucleotides
Theoretical weight: 50.72 KDa
P4-P6 RNA ribozyme domain in PDB entry 1l8v, assembly 1, front view.
P4-P6 RNA ribozyme domain in PDB entry 1l8v, assembly 1, side view.
P4-P6 RNA ribozyme domain in PDB entry 1l8v, assembly 1, top view.
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