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STRUCTURE OF A TERNARY COMPLEX OF AN ALLOSTERIC LACTATE DEHYDROGENASE FROM BACILLUS STEAROTHERMOPHILUS AT 2.5 ANGSTROMS RESOLUTION

X-ray diffraction
2.5Å resolution
PDB entry 1ldn coloured by chain, front view.
PDB entry 1ldn coloured by chain, side view.
PDB entry 1ldn coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: L-lactate dehydrogenase
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Multimeric state: homo tetramer
Accessible surface area: 38697.85 Å2
Buried surface area: 21464.36 Å2
Dissociation area: 3,319.29 Å2
Dissociation energy (ΔGdiss): 29.47 kcal/mol
Dissociation entropy (TΔSdiss): 15.49 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-132419
Assembly 2
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Assembly Name: L-lactate dehydrogenase
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Multimeric state: homo tetramer
Accessible surface area: 38543.76 Å2
Buried surface area: 21267.98 Å2
Dissociation area: 3,280.38 Å2
Dissociation energy (ΔGdiss): 34.23 kcal/mol
Dissociation entropy (TΔSdiss): 15.48 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-132419

Macromolecules

L-lactate dehydrogenase
Chains: A, B, C, D, E, F, G, H
Length: 316 amino acids
Theoretical weight: 34.75 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
UniProt:
  • Canonical: P00344 (Residues: 1-316; Coverage: 100%)
Gene names: lct, ldh
Pfam:
InterPro:
CATH:
SCOP:
L-lactate dehydrogenase in PDB entry 1ldn, assembly 1, front view.
L-lactate dehydrogenase in PDB entry 1ldn, assembly 1, side view.
L-lactate dehydrogenase in PDB entry 1ldn, assembly 1, top view.
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