› 1lhn

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE N-TERMINAL LG-DOMAIN OF SHBG IN COMPLEX WITH 5ALPHA-ANDROSTANE-3BETA,17ALPHA-DIOL

Released:
DOI: 10.2210/pdb1lhn/pdb
PDB entry 1lhn coloured by chain, front view.
PDB entry 1lhn coloured by chain, side view.
PDB entry 1lhn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Steroid ligands bind human sex hormone-binding globulin in specific orientations and produce distinct changes in protein conformation.
Grishkovskaya I, Avvakumov GV, Hammond GL, Catalano MG, Muller YA
J Biol Chem 277 32086-93 (2002)
PMID: 12065592

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sex hormone-binding globulin Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 20.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04278 (Residues: 30-218; Coverage: 51%)
Gene name: SHBG
Sequence domains: Laminin G domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand ZN 1 x ZN
Ligand AON 1 x AON
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: R32
Unit cell:
a: 103.9Å b: 103.9Å c: 84.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.239
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Diverse roles for sex hormone-binding globulin in reproduction.
Hammond GL. (2011)
9 more

1 mention without citation

Proteomic analysis of Col11a1-associated protein complexes.
Brown et al. (2011)