Assemblies
Assembly Name:
T7 RNA polymerase and RNA and DNA
Multimeric state:
hetero tetramer
Accessible surface area:
46441.75 Å2
Buried surface area:
7559.27 Å2
Dissociation area:
1,478.83
Å2
Dissociation energy (ΔGdiss):
9.92
kcal/mol
Dissociation entropy (TΔSdiss):
11.72
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-114202
Macromolecules
Chain: D
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli BL21
UniProt:
Pfam:
InterPro:
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
P00573 (Residues: 1-883; Coverage: 100%)
Pfam:
InterPro:
- DNA/RNA polymerase superfamily
- DNA-directed RNA polymerase, N-terminal domain superfamily
- DNA-directed RNA polymerase, phage-type
- DNA-directed RNA polymerase, N-terminal
- DNA-directed RNA polymerase, helix hairpin domain superfamily
- DNA-directed RNA polymerase, C-terminal domain, phage-type
- DNA-directed RNA polymerase, N-terminal domain
- Helix Hairpins
- Alpha-Beta Plaits
- Helix Hairpins
- 5' to 3' exonuclease, C-terminal subdomain
Name:
Template DNA
Representative chains: T
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Representative chains: T
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Name:
Non-Template DNA
Representative chains: N
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 17 nucleotides
Theoretical weight: 5.19 KDa
Representative chains: N
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 17 nucleotides
Theoretical weight: 5.19 KDa
Name:
Messenger RNA
Representative chains: R
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 10 nucleotides
Theoretical weight: 3.24 KDa
Representative chains: R
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 10 nucleotides
Theoretical weight: 3.24 KDa