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Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase

X-ray diffraction
2.1Å resolution
PDB entry 1msw coloured by chain, front view.
PDB entry 1msw coloured by chain, side view.
PDB entry 1msw coloured by chain, top view.
Source organism: Escherichia phage T7
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: T7 RNA polymerase and RNA and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 46441.75 Å2
Buried surface area: 7559.27 Å2
Dissociation area: 1,478.83 Å2
Dissociation energy (ΔGdiss): 9.92 kcal/mol
Dissociation entropy (TΔSdiss): 11.72 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-114202

Macromolecules

T7 RNA polymerase
Chain: D
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Pfam:
InterPro:
CATH:
SCOP: T7 RNA polymerase
T7 RNA polymerase in PDB entry 1msw, assembly 1, front view.
T7 RNA polymerase in PDB entry 1msw, assembly 1, side view.
T7 RNA polymerase in PDB entry 1msw, assembly 1, top view.
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DNA chain T
Name: Template DNA
Representative chains: T
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Template DNA in PDB entry 1msw, assembly 1, front view.
Template DNA in PDB entry 1msw, assembly 1, side view.
Template DNA in PDB entry 1msw, assembly 1, top view.
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DNA chain N
Name: Non-Template DNA
Representative chains: N
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 17 nucleotides
Theoretical weight: 5.19 KDa
Non-Template DNA in PDB entry 1msw, assembly 1, front view.
Non-Template DNA in PDB entry 1msw, assembly 1, side view.
Non-Template DNA in PDB entry 1msw, assembly 1, top view.
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RNA chain R
Name: Messenger RNA
Representative chains: R
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 10 nucleotides
Theoretical weight: 3.24 KDa
Messenger RNA in PDB entry 1msw, assembly 1, front view.
Messenger RNA in PDB entry 1msw, assembly 1, side view.
Messenger RNA in PDB entry 1msw, assembly 1, top view.
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