› 1nk2

Solution NMR

VND/NK-2 HOMEODOMAIN/DNA COMPLEX, NMR, 20 STRUCTURES

Released:
DOI: 10.2210/pdb1nk2/pdb
PDB entry 1nk2 coloured by chain, ensemble of 20 models, front view.
PDB entry 1nk2 coloured by chain, ensemble of 20 models, side view.
PDB entry 1nk2 coloured by chain, ensemble of 20 models, top view.
Source organism: Drosophila melanogaster
Primary publication:
Interactions of the vnd/NK-2 homeodomain with DNA by nuclear magnetic resonance spectroscopy: basis of binding specificity.
Gruschus JM, Tsao DH, Wang LH, Nirenberg M, Ferretti JA
Biochemistry 36 5372-80 (1997)
PMID: 9154919

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-506307 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeobox protein vnd Chain: P
Molecule details ›
Chain: P
Length: 77 amino acids
Theoretical weight: 9.37 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P22808 (Residues: 537-613; Coverage: 11%)
Gene names: CG6172, NK2, vnd
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*TP*GP*TP*GP*TP*CP*AP*AP*GP*TP*GP*GP*CP*TP*GP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 4.96 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*AP*CP*AP*GP*CP*CP*AP*CP*TP*TP*GP*AP*CP*AP*CP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.84 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY/ SIMULATED ANNEALING
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Intrinsically disordered regions as affinity tuners in protein-DNA interactions.
Vuzman et al. (2012)
9 more

6 mentions without citation

Finding nuclear localization signals.
Cokol et al. (2000)
5 more