› 1ocm

X-ray diffraction
1.9Å resolution

THE CRYSTAL STRUCTURE OF MALONAMIDASE E2 COVALENTLY COMPLEXED WITH PYROPHOSPHATE FROM BRADYRHIZOBIUM JAPONICUM

Released:
DOI: 10.2210/pdb1ocm/pdb
PDB entry 1ocm coloured by chain, front view.
PDB entry 1ocm coloured by chain, side view.
PDB entry 1ocm coloured by chain, top view.
Source organism: Bradyrhizobium japonicum
Primary publication:
Characterization of a novel Ser-cisSer-Lys catalytic triad in comparison with the classical Ser-His-Asp triad.
Shin S, Yun YS, Koo HM, Kim YS, Choi KY, Oh BH
J Biol Chem 278 24937-43 (2003)
PMID: 12711609

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 412 amino acids
Theoretical weight: 43.59 KDa
Source organism: Bradyrhizobium japonicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZIV5 (Residues: 1-412; Coverage: 100%)
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain

Ligands and Environments

1 bound ligand:
Ligand POP 2 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: P21212
Unit cell:
a: 103.265Å b: 94.427Å c: 74.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.222
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure and function of fatty acid amide hydrolase.
McKinney et al. (2005)
4 more

1 mention without citation

X-ray crystallographic analysis of the 6-aminohexanoate cyclic dimer hydrolase: catalytic mechanism and evolution of an enzyme responsible for nylon-6 byproduct degradation.
Yasuhira et al. (2010)