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Structure of Escherichia coli malate synthase G:pyruvate:acetyl-Coenzyme A abortive ternary complex at 1.95 angstrom resolution

X-ray diffraction
1.95Å resolution
PDB entry 1p7t coloured by chain, front view.
PDB entry 1p7t coloured by chain, side view.
PDB entry 1p7t coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. W3110
Assembly composition:
monomeric (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1
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Assembly Name: Malate synthase G
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Multimeric state: monomeric
Accessible surface area: 25043.96 Å2
Buried surface area: 1948.87 Å2
Dissociation area: 183.18 Å2
Dissociation energy (ΔGdiss): -4.17 kcal/mol
Dissociation entropy (TΔSdiss): 1.38 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-153394
Assembly 2 (preferred)
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Assembly Name: Malate synthase G
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Multimeric state: monomeric
Accessible surface area: 24884.95 Å2
Buried surface area: 2685.0 Å2
Dissociation area: 267.7 Å2
Dissociation energy (ΔGdiss): -8.74 kcal/mol
Dissociation entropy (TΔSdiss): 3.21 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-153394

Macromolecules

Malate synthase G
Chain: A
Length: 731 amino acids
Theoretical weight: 81.65 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: P37330 (Residues: 1-723; Coverage: 100%)
Gene names: JW2943, b2976, glc, glcB
Pfam:
InterPro:
CATH:
SCOP: Malate synthase G
Malate synthase G in PDB entry 1p7t, assembly 1, front view.
Malate synthase G in PDB entry 1p7t, assembly 1, side view.
Malate synthase G in PDB entry 1p7t, assembly 1, top view.
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Malate synthase G
Chain: B
Length: 731 amino acids
Theoretical weight: 81.67 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: P37330 (Residues: 1-723; Coverage: 100%)
Gene names: JW2943, b2976, glc, glcB
Pfam:
InterPro:
CATH:
SCOP: Malate synthase G
Malate synthase G in PDB entry 1p7t, assembly 2, front view.
Malate synthase G in PDB entry 1p7t, assembly 2, side view.
Malate synthase G in PDB entry 1p7t, assembly 2, top view.
Molecule details ›

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