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Crystal structure of levansucrase (E342A) complexed with sucrose

X-ray diffraction
2.1Å resolution
PDB entry 1pt2 coloured by chain, front view.
PDB entry 1pt2 coloured by chain, side view.
PDB entry 1pt2 coloured by chain, top view.
Source organism: Bacillus subtilis
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Levansucrase
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Multimeric state: monomeric
Accessible surface area: 16783.31 Å2
Buried surface area: 873.33 Å2
Dissociation area: 324.3 Å2
Dissociation energy (ΔGdiss): -5 kcal/mol
Dissociation entropy (TΔSdiss): 4.15 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-138729

Macromolecules

Levansucrase
Chain: A
Length: 447 amino acids
Theoretical weight: 50.26 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05655 (Residues: 30-473; Coverage: 100%)
Gene names: BSU34450, sacB
Pfam: Levansucrase/Invertase
InterPro:
CATH: Glycosyl hydrolase domain; family 43
SCOP: Levansucrase
Levansucrase in PDB entry 1pt2, assembly 1, front view.
Levansucrase in PDB entry 1pt2, assembly 1, side view.
Levansucrase in PDB entry 1pt2, assembly 1, top view.
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