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Crystal structures of the Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase-GMP and -IMP complexes: comparison of purine binding interactions with the XMP complex.

Héroux A, White EL, Ross LJ, Borhani DW
Biochemistry 38 14485-94 (1999)
Related entries: 1qk3, 1qk5

Cited: 29 times
EuropePMC logo PMID: 10545170

Abstract

The crystal structures of the guanosine 5'-monophosphate (GMP) and inosine 5'-monophosphate (IMP) complexes of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolution. These complexes, which crystallize in space groups P2(1) (a = 65.45 A, b = 90.84 A, c = 80. 26 A, and beta = 92.53 degrees ) and P2(1)2(1)2(1) (a = 84.54 A, b = 102.44 A, and c = 108.83 A), each comprise a tetramer in the crystallographic asymmetric unit. All active sites in the tetramers are fully occupied by the nucleotide. Comparison of these structures with that of the xanthosine 5'-monophosphate (XMP)-pyrophosphate-Mg(2+) ternary complex reported in the following article [Héroux, A., et al. (1999) Biochemistry 38, 14495-14506] shows how T. gondii HGPRT is able to recognize guanine, hypoxanthine, and xanthine as substrates, and suggests why the human enzyme cannot use xanthine efficiently. Comparison with the apoenzyme reveals the structural changes that occur upon binding of purines and ribose 5'-phosphate to HGPRT. Two structural features important to the HGPRT mechanism, a previously unrecognized active site loop (loop III', residues 180-184) and an active site peptide bond (Leu78-Lys79) that adopts both the cis and the trans configurations, are presented.

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  2. Structures of hypoxanthine-guanine phosphoribosyltransferase (TTHA0220) from Thermus thermophilus HB8. Kanagawa M, Baba S, Ebihara A, Shinkai A, Hirotsu K, Mega R, Kim K, Kuramitsu S, Sampei G, Kawai G. Acta Crystallogr Sect F Struct Biol Cryst Commun 66 893-898 (2010)


Articles citing this publication (27)

  1. Substrate deformation in a hypoxanthine-guanine phosphoribosyltransferase ternary complex: the structural basis for catalysis. Héroux A, White EL, Ross LJ, Kuzin AP, Borhani DW. Structure 8 1309-1318 (2000)
  2. Structural and functional analysis of mutations at the human hypoxanthine phosphoribosyl transferase (HPRT1) locus. Duan J, Nilsson L, Lambert B. Hum Mutat 23 599-611 (2004)
  3. The crystal structure of free human hypoxanthine-guanine phosphoribosyltransferase reveals extensive conformational plasticity throughout the catalytic cycle. Keough DT, Brereton IM, de Jersey J, Guddat LW. J Mol Biol 351 170-181 (2005)
  4. Differential localization of alternatively spliced hypoxanthine-xanthine-guanine phosphoribosyltransferase isoforms in Toxoplasma gondii. Chaudhary K, Donald RG, Nishi M, Carter D, Ullman B, Roos DS. J Biol Chem 280 22053-22059 (2005)
  5. Structural mechanism governing cis and trans isomeric states and an intramolecular switch for cis/trans isomerization of a non-proline peptide bond observed in crystal structures of scorpion toxins. Guan RJ, Xiang Y, He XL, Wang CG, Wang M, Zhang Y, Sundberg EJ, Wang DC. J Mol Biol 341 1189-1204 (2004)
  6. Crystal structure of fully ligated adenylosuccinate synthetase from Plasmodium falciparum. Eaazhisai K, Jayalakshmi R, Gayathri P, Anand RP, Sumathy K, Balaram H, Murthy MR. J Mol Biol 335 1251-1264 (2004)
  7. Interactions at the dimer interface influence the relative efficiencies for purine nucleotide synthesis and pyrophosphorolysis in a phosphoribosyltransferase. Canyuk B, Medrano FJ, Wenck MA, Focia PJ, Eakin AE, Craig SP. J Mol Biol 335 905-921 (2004)
  8. Virtual screening of combinatorial libraries across a gene family: in search of inhibitors of Giardia lamblia guanine phosphoribosyltransferase. Aronov AM, Munagala NR, Kuntz ID, Wang CC. Antimicrob Agents Chemother 45 2571-2576 (2001)
  9. Unusual substrate specificity of a chimeric hypoxanthine-guanine phosphoribosyltransferase containing segments from the Plasmodium falciparum and human enzymes. Sujay Subbayya IN, Sukumaran S, Shivashankar K, Balaram H. Biochem Biophys Res Commun 272 596-602 (2000)
  10. Evaluation of the Trypanosoma brucei 6-oxopurine salvage pathway as a potential target for drug discovery. Doleželová E, Terán D, Gahura O, Kotrbová Z, Procházková M, Keough D, Špaček P, Hocková D, Guddat L, Zíková A. PLoS Negl Trop Dis 12 e0006301 (2018)
  11. Plasmodium falciparum hypoxanthine guanine phosphoribosyltransferase. Stability studies on the product-activated enzyme. Raman J, Ashok CS, Subbayya SI, Anand RP, Selvi ST, Balaram H. FEBS J 272 1900-1911 (2005)
  12. Evidence for multiple active states of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase. Sujay Subbayya IN, Balaram H. Biochem Biophys Res Commun 279 433-437 (2000)
  13. Pyrophosphate activation in hypoxanthine--guanine phosphoribosyltransferase with transition state analogue. Deng H, Callender R, Schramm VL, Grubmeyer C. Biochemistry 49 2705-2714 (2010)
  14. A non-active site mutation in human hypoxanthine guanine phosphoribosyltransferase expands substrate specificity. Raman J, Sumathy K, Anand RP, Balaram H. Arch Biochem Biophys 427 116-122 (2004)
  15. Alternative IMP binding in feedback inhibition of hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcongensis. Chen Q, Liang Y, Su X, Gu X, Zheng X, Luo M. J Mol Biol 348 1199-1210 (2005)
  16. Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin. Garcia-Pino A, Buts L, Wyns L, Loris R. J Mol Biol 361 153-167 (2006)
  17. Crystal structures and inhibition of Trypanosoma brucei hypoxanthine-guanine phosphoribosyltransferase. Terán D, Hocková D, Česnek M, Zíková A, Naesens L, Keough DT, Guddat LW. Sci Rep 6 35894 (2016)
  18. Crystal structure of Leishmania tarentolae hypoxanthine-guanine phosphoribosyltransferase. Monzani PS, Trapani S, Thiemann OH, Oliva G. BMC Struct Biol 7 59 (2007)
  19. Two enzymes, TilS and HprT, can form a complex to function as a transcriptional activator for the cell division protease gene ftsH in Bacillus subtilis. Lin TH, Hu YN, Shaw GC. J Biochem 155 5-16 (2014)
  20. Functional significance of four successive glycine residues in the pyrophosphate binding loop of fungal 6-oxopurine phosphoribosyltransferases. Moynié L, Giraud MF, Breton A, Boissier F, Daignan-Fornier B, Dautant A. Protein Sci 21 1185-1196 (2012)
  21. Structure of Salmonella typhimurium OMP synthase in a complete substrate complex. Grubmeyer C, Hansen MR, Fedorov AA, Almo SC. Biochemistry 51 4397-4405 (2012)
  22. Acidic residues in the purine binding site govern the 6-oxopurine specificity of the Leishmania donovani xanthine phosphoribosyltransferase. Ullman B, Cyr N, Choi K, Jardim A. Int J Biochem Cell Biol 42 253-262 (2010)
  23. Crystal structure of a chimera of human and Plasmodium falciparum hypoxanthine guanine phosphoribosyltransferases provides insights into oligomerization. Gayathri P, Sujay Subbayya IN, Ashok CS, Selvi TS, Balaram H, Murthy MR. Proteins 73 1010-1020 (2008)
  24. Slow ligand-induced conformational switch increases the catalytic rate in Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase. Roy S, Karmakar T, Prahlada Rao VS, Nagappa LK, Balasubramanian S, Balaram H. Mol Biosyst 11 1410-1424 (2015)
  25. Differential Distortion of Purine Substrates by Human and Plasmodium falciparum Hypoxanthine-Guanine Phosphoribosyltransferase to Catalyse the Formation of Mononucleotides. Karnawat V, Gogia S, Balaram H, Puranik M. Chemphyschem 16 2172-2181 (2015)
  26. Role of W181 in modulating kinetic properties of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase. Roy S, Karmakar T, Nagappa LK, Prahlada Rao VS, Balasubramanian S, Balaram H. Proteins 84 1658-1669 (2016)
  27. Structural and dynamical correlations in PfHGXPRT oligomers: A molecular dynamics simulation study. Karmakar T, Roy S, Balaram H, Balasubramanian S. J Biomol Struct Dyn 34 1590-1605 (2016)


Related citations provided by authors (2)

  1. Crystal structure of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase with XMP, pyrophosphate, and two Mg(2+) ions bound: insights into the catalytic mechanism.. Héroux A, White EL, Ross LJ, Davis RL, Borhani DW Biochemistry 38 14495-506 (1999)
  2. Isolation and sequencing of a cDNA encoding the hypoxanthine-guanine phosphoribosyltransferase from Toxoplasma gondii.. Vasanthakumar G, van Ginkel S, Parish G Gene 147 153-4 (1994)

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