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Crystal Structure of the Heterodimeric Ecdysone Receptor DNA-binding Complex

X-ray diffraction
2.24Å resolution
PDB entry 1r0o coloured by chain, front view.
PDB entry 1r0o coloured by chain, side view.
PDB entry 1r0o coloured by chain, top view.
Source organism: Drosophila melanogaster
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Protein ultraspiracle and Ecdysone receptor and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 13400.08 Å2
Buried surface area: 5294.52 Å2
Dissociation area: 1,103.3 Å2
Dissociation energy (ΔGdiss): 2.84 kcal/mol
Dissociation entropy (TΔSdiss): 11.17 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-114485

Macromolecules

Protein ultraspiracle
Chain: A
Length: 86 amino acids
Theoretical weight: 10.09 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20153 (Residues: 94-179; Coverage: 17%)
Gene names: CG4380, Cf1, NR2B4, usp
Pfam: Zinc finger, C4 type (two domains)
InterPro:
CATH: Erythroid Transcription Factor GATA-1, subunit A
SCOP: Nuclear receptor
Protein ultraspiracle in PDB entry 1r0o, assembly 1, front view.
Protein ultraspiracle in PDB entry 1r0o, assembly 1, side view.
Protein ultraspiracle in PDB entry 1r0o, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P20153  
1861020304050607080
 
20406080NHPLSGSKHLCSICGDRASGKHYGVYSCEGCKGFFKRTVRKDLTYACRENRNCIIDKRQRNRCQYCRYQKCLTCGMKREAVQEERQ
UniProt
P20153
Chains
Domains
Secondary structure
Flexibility predictions
Early folding residue predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
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Ecdysone receptor
Chain: B
Length: 109 amino acids
Theoretical weight: 12.55 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34021 (Residues: 256-364; Coverage: 12%)
Gene names: CG1765, EcR, NR1H1
Pfam: Zinc finger, C4 type (two domains)
InterPro:
CATH: Erythroid Transcription Factor GATA-1, subunit A
SCOP: Nuclear receptor
Ecdysone receptor in PDB entry 1r0o, assembly 1, front view.
Ecdysone receptor in PDB entry 1r0o, assembly 1, side view.
Ecdysone receptor in PDB entry 1r0o, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P34021  
1109102030405060708090100
 
50100
UniProt
P34021
Chains
Domains
Secondary structure
Flexibility predictions
Early folding residue predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
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DNA chain C
Name: Ecdysone Response Element
Representative chains: C
Length: 18 nucleotides
Theoretical weight: 5.5 KDa
Ecdysone Response Element in PDB entry 1r0o, assembly 1, front view.
Ecdysone Response Element in PDB entry 1r0o, assembly 1, side view.
Ecdysone Response Element in PDB entry 1r0o, assembly 1, top view.
11824681012141618
 
51015CCGAGGTCAATGACCTCG
Chains
RSRZ Outlier Chain A (auth C)
Chain A (auth C)
Interaction interfaces
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DNA chain D
Name: Ecdysone Response Element
Representative chains: D
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
Ecdysone Response Element in PDB entry 1r0o, assembly 1, front view.
Ecdysone Response Element in PDB entry 1r0o, assembly 1, side view.
Ecdysone Response Element in PDB entry 1r0o, assembly 1, top view.
11824681012141618
 
51015CCGAGGTCATTGACCTCG
Chains
RSRZ Outlier Chain B (auth D)
Chain B (auth D)
Interaction interfaces
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