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Crystal structure of mutant T33A of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform, complexed with NADP

X-ray diffraction
2.2Å resolution
PDB entry 1rm3 coloured by chain, front view.
PDB entry 1rm3 coloured by chain, side view.
PDB entry 1rm3 coloured by chain, top view.
Source organism: Spinacia oleracea
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
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Multimeric state: homo tetramer
Accessible surface area: 43857.69 Å2
Buried surface area: 21826.67 Å2
Dissociation area: 4,683.33 Å2
Dissociation energy (ΔGdiss): 82.74 kcal/mol
Dissociation entropy (TΔSdiss): 16.09 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-148772
Assembly 2
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Assembly Name: Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
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Multimeric state: homo tetramer
Accessible surface area: 43904.65 Å2
Buried surface area: 22318.01 Å2
Dissociation area: 4,635.97 Å2
Dissociation energy (ΔGdiss): 79.56 kcal/mol
Dissociation entropy (TΔSdiss): 16.08 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-148772

Macromolecules

Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
Chains: A, B, O
Length: 337 amino acids
Theoretical weight: 36.23 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19866 (Residues: 66-401; Coverage: 84%)
Gene names: GAPA, GPA1
Pfam:
InterPro:
CATH:
SCOP:
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic in PDB entry 1rm3, assembly 1, front view.
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic in PDB entry 1rm3, assembly 1, side view.
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic in PDB entry 1rm3, assembly 1, top view.
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