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TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX

X-ray diffraction
3.58Å resolution
PDB entry 1sa0 coloured by chain, front view.
PDB entry 1sa0 coloured by chain, side view.
PDB entry 1sa0 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero pentamer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 64929.51 Å2
Buried surface area: 19072.36 Å2
Dissociation area: 3,050.26 Å2
Dissociation energy (ΔGdiss): 23.12 kcal/mol
Dissociation entropy (TΔSdiss): 15.23 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-158932

Macromolecules

Tubulin alpha-1D chain
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.16 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2HJ86 (Residues: 1-452; Coverage: 100%)
Gene name: TUBA1D
Pfam:
InterPro:
CATH:
SCOP:
Tubulin alpha-1D chain in PDB entry 1sa0, assembly 1, front view.
Tubulin alpha-1D chain in PDB entry 1sa0, assembly 1, side view.
Tubulin alpha-1D chain in PDB entry 1sa0, assembly 1, top view.
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Tubulin beta-2B chain
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Pfam:
InterPro:
CATH:
SCOP:
Tubulin beta-2B chain in PDB entry 1sa0, assembly 1, front view.
Tubulin beta-2B chain in PDB entry 1sa0, assembly 1, side view.
Tubulin beta-2B chain in PDB entry 1sa0, assembly 1, top view.
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Stathmin-4
Chain: E
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63043 (Residues: 48-189; Coverage: 75%)
Gene name: Stmn4
Pfam: Stathmin family
InterPro:
CATH: Methane Monooxygenase Hydroxylase; Chain G, domain 1
SCOP: Stathmin
Stathmin-4 in PDB entry 1sa0, assembly 1, front view.
Stathmin-4 in PDB entry 1sa0, assembly 1, side view.
Stathmin-4 in PDB entry 1sa0, assembly 1, top view.
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