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REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION

Electron Microscopy
7.6Å resolution
PDB entry 1uon coloured by chain, front view.
PDB entry 1uon coloured by chain, side view.
PDB entry 1uon coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct RNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: RNA-directed RNA polymerase and RNA
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Multimeric state: hetero trimer
Accessible surface area: 46157.83 Å2
Buried surface area: 6345.45 Å2
Dissociation area: 980.15 Å2
Dissociation energy (ΔGdiss): 16.95 kcal/mol
Dissociation entropy (TΔSdiss): 8.35 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-186538

Macromolecules

RNA-directed RNA polymerase
Chain: A
Length: 1267 amino acids
Theoretical weight: 142.42 KDa
Source organism: Reovirus sp.
UniProt:
  • Canonical: Q8V1A3 (Residues: 1-121; Coverage: 100%)
Pfam: Reovirus RNA-dependent RNA polymerase lambda 3
InterPro:
SCOP: Reovirus components
RNA-directed RNA polymerase in PDB entry 1uon, assembly 1, front view.
RNA-directed RNA polymerase in PDB entry 1uon, assembly 1, side view.
RNA-directed RNA polymerase in PDB entry 1uon, assembly 1, top view.
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RNA chain B
Name: 5'-R(*GP*GP*GP*GP*GP*)-3'
Representative chains: B
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 5 nucleotides
Theoretical weight: 1.68 KDa
5'-R(*GP*GP*GP*GP*GP*)-3' in PDB entry 1uon, assembly 1, front view.
5'-R(*GP*GP*GP*GP*GP*)-3' in PDB entry 1uon, assembly 1, side view.
5'-R(*GP*GP*GP*GP*GP*)-3' in PDB entry 1uon, assembly 1, top view.
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RNA chain C
Name: 5'-R(*UP*AP*GP*CP*CP*CP*CP*CP*)-3'
Representative chains: C
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 8 nucleotides
Theoretical weight: 2.46 KDa
5'-R(*UP*AP*GP*CP*CP*CP*CP*CP*)-3' in PDB entry 1uon, assembly 1, front view.
5'-R(*UP*AP*GP*CP*CP*CP*CP*CP*)-3' in PDB entry 1uon, assembly 1, side view.
5'-R(*UP*AP*GP*CP*CP*CP*CP*CP*)-3' in PDB entry 1uon, assembly 1, top view.
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