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E.coli FolC in complex with ADP, without folate substrate

Source organism: Escherichia coli
Biochemical function: guanosine tetraphosphate binding
Biological process: tetrahydrofolylpolyglutamate biosynthetic process
Cellular component: cytoplasm

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EC 6.3.2.17: Tetrahydrofolate synthase

Reaction catalysed:
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
Systematic name:
Tetrahydropteroyl-(gamma-polyglutamate):L-glutamate gamma-ligase (ADP-forming)
Alternative Name(s):
  • FPGS
  • Folate polyglutamate synthetase
  • Folylpoly(gamma-glutamate) synthase
  • Folylpoly-gamma-glutamate synthase
  • Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase
  • Folylpolyglutamate synthase
  • Folylpolyglutamate synthetase
  • Folylpolyglutamyl synthetase
  • Formyltetrahydropteroyldiglutamate synthetase
  • N(10)-formyltetrahydropteroyldiglutamate synthetase
  • Tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming)
  • Tetrahydrofolyl-(gamma-Glu)(n):L-glutamate gamma-ligase (ADP-forming)
  • Tetrahydrofolylpolyglutamate synthase

EC 6.3.2.12: Dihydrofolate synthase

Reaction catalysed:
ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate
Systematic name:
7,8-dihydropteroate:L-glutamate ligase (ADP-forming)
Alternative Name(s):
  • 7,8-dihydrofolate synthetase
  • 7,8-dihydropteroate:L-glutamate ligase (ADP)
  • DHFS
  • Dihydrofolate synthetase
  • Dihydropteroate:L-glutamate ligase (ADP-forming)
  • H(2)-folate synthetase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF02875
Domain description: Mur ligase, glutamate ligase domain
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase
The deposited structure of PDB entry 1w7k contains 1 copy of Pfam domain PF02875 (Mur ligase family, glutamate ligase domain) in Dihydrofolate synthase/folylpolyglutamate synthase. Showing 1 copy in chain A.

PF08245
Domain description: Mur ligase middle domain
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase
The deposited structure of PDB entry 1w7k contains 1 copy of Pfam domain PF08245 (Mur ligase middle domain) in Dihydrofolate synthase/folylpolyglutamate synthase. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR001645
Domain description: Folylpolyglutamate synthetase
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase
IPR036615
Domain description: Mur ligase, C-terminal domain superfamily
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase
IPR018109
Domain description: Folylpolyglutamate synthetase, conserved site
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase
IPR036565
Domain description: Mur-like, catalytic domain superfamily
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase
IPR004101
Domain description: Mur ligase, C-terminal
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase
IPR013221
Domain description: Mur ligase, central
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase

Structure domains

CATH CATH domains
3.90.190.20
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Protein-Tyrosine Phosphatase; Chain A
Homology: Mur ligase, C-terminal domain
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase
The deposited structure of PDB entry 1w7k contains 1 copy of CATH domain 3.90.190.20 (Protein-Tyrosine Phosphatase; Chain A) in Dihydrofolate synthase/folylpolyglutamate synthase. Showing 1 copy in chain A.
3.40.1190.10
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
Homology: Mur-like, catalytic domain
Occurring in:
  1. Dihydrofolate synthase/folylpolyglutamate synthase
The deposited structure of PDB entry 1w7k contains 1 copy of CATH domain 3.40.1190.10 (UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase) in Dihydrofolate synthase/folylpolyglutamate synthase. Showing 1 copy in chain A.

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