› 1xuz

X-ray diffraction
2.2Å resolution

Crystal structure analysis of sialic acid synthase (NeuB)from Neisseria meningitidis, bound to Mn2+, Phosphoenolpyruvate, and N-acetyl mannosaminitol

Released:
DOI: 10.2210/pdb1xuz/pdb
PDB entry 1xuz coloured by chain, front view.
PDB entry 1xuz coloured by chain, side view.
PDB entry 1xuz coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Structural and mechanistic analysis of sialic acid synthase NeuB from Neisseria meningitidis in complex with Mn2+, phosphoenolpyruvate, and N-acetylmannosaminitol.
Gunawan J, Simard D, Gilbert M, Lovering AL, Wakarchuk WW, Tanner ME, Strynadka NC
J Biol Chem 280 3555-63 (2005)
PMID: 15516336

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AFP-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 38.4 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q57265 (Residues: 1-349; Coverage: 100%)
Gene names: A6J53_03590, DE8555_0059, NCTC8554_01562, siaC, synC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MMN 1 x MMN
Ligand MN 1 x MN
Ligand PEP 1 x PEP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 58.531Å b: 75.654Å c: 77.866Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.248
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

The enzymes of sialic acid biosynthesis.
Tanner ME. (2005)
11 more

8 mentions without citation

Attenuation of Streptococcus suis virulence by the alteration of bacterial surface architecture.
Feng et al. (2012)
7 more