› 1y9t

X-ray diffraction
1.87Å resolution

Crystal structure of a type III secretion system protein complexed with the lipid, 1-monohexanoyl-2-hydroxy-sn-glycero-3-phosphate

Released:
DOI: 10.2210/pdb1y9t/pdb
PDB entry 1y9t coloured by chain, front view.
PDB entry 1y9t coloured by chain, side view.
PDB entry 1y9t coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Structure and biochemical analysis of a secretin pilot protein.
Lario PI, Pfuetzner RA, Frey EA, Creagh L, Haynes C, Maurelli AT, Strynadka NC
EMBO J 24 1111-21 (2005)
PMID: 15775974

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141040 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 3 secretion system pilotin Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.9 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A1X2 (Residues: 28-142; Coverage: 97%)
Gene names: CP0143, mxiM, sctG
Sequence domains: Pilot protein MxiM
Structure domains: Pilot protein MxiM

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand HHG 1 x HHG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 83.036Å b: 49.721Å c: 29.52Å
α: 90° β: 101.99° γ: 90°
R-values:
R R work R free
0.208 0.206 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
18 more

4 mentions without citation

Structure and biochemical analysis of a secretin pilot protein.
Lario et al. (2005)
3 more