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Crystal structure of a type III secretion system protein complexed with the lipid, 1-monohexanoyl-2-hydroxy-sn-glycero-3-phosphate

X-ray diffraction
1.87Å resolution
PDB entry 1y9t coloured by chain, front view.
PDB entry 1y9t coloured by chain, side view.
PDB entry 1y9t coloured by chain, top view.
Source organism: Shigella flexneri
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Type 3 secretion system pilotin
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Multimeric state: monomeric
Accessible surface area: 6708.95 Å2
Buried surface area: 778.98 Å2
Dissociation area: 317.27 Å2
Dissociation energy (ΔGdiss): -6.62 kcal/mol
Dissociation entropy (TΔSdiss): 3.95 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-141040

Macromolecules

Type 3 secretion system pilotin
Chain: A
Length: 115 amino acids
Theoretical weight: 12.9 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A1X2 (Residues: 28-142; Coverage: 97%)
Gene names: CP0143, mxiM, sctG
Pfam: Pilot protein MxiM
InterPro:
CATH: Pilot protein MxiM
Type 3 secretion system pilotin in PDB entry 1y9t, assembly 1, front view.
Type 3 secretion system pilotin in PDB entry 1y9t, assembly 1, side view.
Type 3 secretion system pilotin in PDB entry 1y9t, assembly 1, top view.
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