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SUBTILISIN BPN' 8397+1 (E.C. 3.4.21.14) (MUTANT WITH MET 50 REPLACED BY PHE, ASN 76 REPLACED BY ASP, GLY 169 REPLACED BY ALA, GLN 206 REPLACED BY CYS, ASN 218 REPLACED BY SER AND LYS 256 REPLACED BY TYR) (M50F, N76D, G169A, Q206C, N218S, AND K256Y) IN 20% DIMETHYLFORMAMIDE

X-ray diffraction
1.8Å resolution
PDB entry 1yja coloured by chain, front view.
PDB entry 1yja coloured by chain, side view.
PDB entry 1yja coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Subtilisin BPN'
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Multimeric state: monomeric
PDBe Complex ID: PDB-CPX-133530

Macromolecules

Subtilisin BPN'
Chain: A
Length: 275 amino acids
Theoretical weight: 27.58 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P00782 (Residues: 108-382; Coverage: 78%)
Gene name: apr
Pfam: Subtilase family
InterPro:
CATH: Peptidase S8/S53 domain
SCOP: Subtilases
Subtilisin BPN' in PDB entry 1yja, assembly 1, front view.
Subtilisin BPN' in PDB entry 1yja, assembly 1, side view.
Subtilisin BPN' in PDB entry 1yja, assembly 1, top view.
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