› 1zxm

X-ray diffraction
1.87Å resolution

Human Topo IIa ATPase/AMP-PNP

Released:
DOI: 10.2210/pdb1zxm/pdb
PDB entry 1zxm coloured by chain, front view.
PDB entry 1zxm coloured by chain, side view.
PDB entry 1zxm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nucleotide-dependent domain movement in the ATPase domain of a human type IIA DNA topoisomerase.
Wei H, Ruthenburg AJ, Bechis SK, Verdine GL
J Biol Chem 280 37041-7 (2005)
PMID: 16100112

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145812 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2-alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 45.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11388 (Residues: 29-428; Coverage: 26%)
Gene names: TOP2, TOP2A
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 70.772Å b: 75.285Å c: 164.887Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.243
Expression system: Escherichia coli

Quick links

Citations

15 review citations

DNA topoisomerase II and its growing repertoire of biological functions.
Nitiss JL. (2009)
14 more

78 mentions without citation

Recent advances in the pharmacological diversification of quinazoline/quinazolinone hybrids.
Auti et al. (2020)
77 more