› 2iu5

X-ray diffraction
1.6Å resolution

Dihydroxyacetone kinase operon activator DhaS

Released:
DOI: 10.2210/pdb2iu5/pdb
Model geometry
Fit model/data
PDB entry 2iu5 coloured by chain, front view.
PDB entry 2iu5 coloured by chain, side view.
PDB entry 2iu5 coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis Il1403
Primary publication:
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators.
Christen S, Srinivas A, Bähler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B
J Biol Chem 281 23129-37 (2006)
PMID: 16760471

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type dhaKLM operon transcriptional activator DhaS Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 23.57 KDa
Source organism: Lactococcus lactis subsp. lactis Il1403
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CIV9 (Residues: 1-187; Coverage: 100%)
Gene names: L45062, LL0247, dhaR, dhaS
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 44.459Å b: 46.284Å c: 171.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 citation in other articles

The novel transcriptional regulator SczA mediates protection against Zn2+ stress by activation of the Zn2+-resistance gene czcD in Streptococcus pneumoniae.
Kloosterman et al. (2007)
26 more

1 mention without citation

The TetR family of regulators.
Cuthbertson et al. (2013)

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO