› 2j28

Electron Microscopy
9.5Å resolution

MODEL OF E. COLI SRP BOUND TO 70S RNCS

Released:
DOI: 10.2210/pdb2j28/pdb
PDB entry 2j28 coloured by chain, front view.
PDB entry 2j28 coloured by chain, side view.
PDB entry 2j28 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Following the signal sequence from ribosomal tunnel exit to signal recognition particle.
Halic M, Blau M, Becker T, Mielke T, Pool MR, Wild K, Sinning I, Beckmann R
Nature 444 507-11 (2006)
PMID: 17086193
Related structures: EMD-1261

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero 34-mer (preferred)
PDBe Complex ID:
PDB-CPX-130261 (preferred)
Entry contents:
31 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (34 distinct):
Large ribosomal subunit protein bL32 Chain: 0
Molecule details ›
Chain: 0
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A7N4 (Residues: 2-57; Coverage: 98%)
Gene names: JW1075, b1089, rpmF
Sequence domains: Ribosomal L32p protein family
Large ribosomal subunit protein bL33 Chain: 1
Molecule details ›
Chain: 1
Length: 54 amino acids
Theoretical weight: 6.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A7N9 (Residues: 2-55; Coverage: 98%)
Gene names: JW3611, b3636, rpmG
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: 2
Large ribosomal subunit protein bL35 Chain: 3
Large ribosomal subunit protein bL36A Chain: 4
Rhodopsin Chain: 7
Signal recognition particle protein Chain: 9
Large ribosomal subunit protein uL2 Chain: C
Large ribosomal subunit protein uL3 Chain: D
Large ribosomal subunit protein uL4 Chain: E
Large ribosomal subunit protein uL5 Chain: F
Large ribosomal subunit protein uL6 Chain: G
Large ribosomal subunit protein bL9 Chain: H
Large ribosomal subunit protein uL11 Chain: I
Large ribosomal subunit protein uL13 Chain: J
Large ribosomal subunit protein uL14 Chain: K
Large ribosomal subunit protein uL15 Chain: L
Large ribosomal subunit protein uL16 Chain: M
Large ribosomal subunit protein bL17 Chain: N
Large ribosomal subunit protein uL18 Chain: O
Large ribosomal subunit protein bL19 Chain: P
Large ribosomal subunit protein bL20 Chain: Q
Large ribosomal subunit protein bL21 Chain: R
Large ribosomal subunit protein uL22 Chain: S
Large ribosomal subunit protein uL23 Chain: T
Large ribosomal subunit protein uL24 Chain: U
Large ribosomal subunit protein bL25 Chain: V
Large ribosomal subunit protein bL27 Chain: W
Large ribosomal subunit protein uL29 Chain: X
Large ribosomal subunit protein uL30 Chain: Y
Large ribosomal subunit protein bL31 Chain: Z
4.5S SIGNAL RECOGNITION PARTICLE RNA Chain: 8
5S ribosomal RNA Chain: A
23S ribosomal RNA Chain: B

Ligands and Environments

1 bound ligand:
Ligand MG 111 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 9.5Å
Relevant EMDB volumes: EMD-1261
Expression system: Escherichia coli K-12

Quick links

Citations

40 review citations

Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes.
Rapoport TA. (2007)
39 more

8 mentions without citation

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
7 more