› 2qqw

X-ray diffraction
2.8Å resolution

Crystal structure of a cell-wall invertase (D23A) from Arabidopsis thaliana in complex with sucrose

Released:
DOI: 10.2210/pdb2qqw/pdb
PDB entry 2qqw coloured by chain, front view.
PDB entry 2qqw coloured by chain, side view.
PDB entry 2qqw coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose.
Lammens W, Le Roy K, Van Laere A, Rabijns A, Van den Ende W
J Mol Biol 377 378-85 (2008)
PMID: 18258263

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-559334 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Beta-fructofuranosidase, insoluble isoenzyme CWINV1 Chain: A
Molecule details ›
Chain: A
Length: 537 amino acids
Theoretical weight: 61.06 KDa
Source organism: Arabidopsis thaliana
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q43866 (Residues: 48-584; Coverage: 97%)
Gene names: At3g13790, BFRUCT1, CWINV1, MMM17.26
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P32
Unit cell:
a: 105.877Å b: 105.877Å c: 50.479Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.188 0.252
Expression system: Komagataella pastoris

Quick links

Citations

3 review citations

Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications.
Lammens et al. (2009)
2 more

3 mentions without citation

Structural insights into the pH-controlled targeting of plant cell-wall invertase by a specific inhibitor protein.
Hothorn et al. (2010)
2 more