› 2rhk

X-ray diffraction
1.95Å resolution

Crystal structure of influenza A NS1A protein in complex with F2F3 fragment of human cellular factor CPSF30, Northeast Structural Genomics Targets OR8C and HR6309A

Released:
DOI: 10.2210/pdb2rhk/pdb
PDB entry 2rhk coloured by chain, front view.
PDB entry 2rhk coloured by chain, side view.
PDB entry 2rhk coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for suppression of a host antiviral response by influenza A virus.
Das K, Ma LC, Xiao R, Radvansky B, Aramini J, Zhao L, Marklund J, Kuo RL, Twu KY, Arnold E, Krug RM, Montelione GT
Proc Natl Acad Sci U S A 105 13093-8 (2008)
PMID: 18725644

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero hexamer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131968 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Non-structural protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 15.96 KDa
Source organism: Influenza A virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03495 (Residues: 85-215; Coverage: 55%)
Gene name: NS
Sequence domains: Influenza non-structural protein (NS1)
Structure domains: Influenza virus non-structural protein, effector domain
Cleavage and polyadenylation specificity factor subunit 4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 72 amino acids
Theoretical weight: 8.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95639 (Residues: 61-121; Coverage: 23%)
Gene names: CPSF30, CPSF4, NAR, NEB1
Sequence domains: Zinc-finger containing family
Structure domains: Zinc finger, CCCH-type

Ligands and Environments

3 bound ligands:
Ligand NO3 5 x NO3
Ligand ZN 4 x ZN
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, null
Spacegroup: P41
Unit cell:
a: 50.96Å b: 50.96Å c: 205.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.234
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Structures of influenza A proteins and insights into antiviral drug targets.
Das et al. (2010)
27 more

25 mentions without citation

Innate immune evasion strategies of influenza viruses.
Hale et al. (2010)
24 more