› 2v3g

X-ray diffraction
1.2Å resolution

Structure of a family 26 lichenase in complex with noeuromycin

Released:
DOI: 10.2210/pdb2v3g/pdb
PDB entry 2v3g coloured by chain, front view.
PDB entry 2v3g coloured by chain, side view.
PDB entry 2v3g coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Entry authors: Meloncelli PJ, Gloster TM, Money VA, Tarling CA, Davies GJ, Withers SG, Stick RV

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-538244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase H Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 32.17 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16218 (Residues: 25-305; Coverage: 31%)
Gene names: Cthe_1472, celH
Sequence domains: Glycosyl hydrolase family 26
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand BGC 1 x BGC
Ligand NOY 1 x NOY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 49.765Å b: 63.516Å c: 78.235Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 0.116 0.144
Expression system: Escherichia coli BL21(DE3)

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