› 2zac

X-ray diffraction
1.5Å resolution

Crystal Structure of Family 7 Alginate Lyase A1-II' Y284F in Complex with Product (MMG)

Released:
DOI: 10.2210/pdb2zac/pdb
PDB entry 2zac coloured by chain, front view.
PDB entry 2zac coloured by chain, side view.
PDB entry 2zac coloured by chain, top view.
Source organism: Sphingomonas sp. A1
Entry authors: Ogura K, Yamasaki M, Mikami B, Hashimoto W, Murata K

Function and Biology Details

Reaction catalysed: 
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-565295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alginate lyase 2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 25.78 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WP3 (Residues: 81-308; Coverage: 74%)
Gene name: aly
Sequence domains: Alginate lyase
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: LGU, BEM
Ligand LGU 1 x LGU
Ligand BEM 2 x BEM
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 34.935Å b: 68.884Å c: 80.433Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.191
Expression system: Escherichia coli

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