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Crystal Structure of Family 7 Alginate Lyase A1-II' Y284F in Complex with Product (MMG)

X-ray diffraction
1.5Å resolution
PDB entry 2zac coloured by chain, front view.
PDB entry 2zac coloured by chain, side view.
PDB entry 2zac coloured by chain, top view.
Source organism: Sphingomonas sp. A1
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Alginate lyase 2 domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 9101.33 Å2
Buried surface area: 1372.55 Å2
Dissociation area: 102.04 Å2
Dissociation energy (ΔGdiss): 0.62 kcal/mol
Dissociation entropy (TΔSdiss): 0.87 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-565295

Macromolecules

Alginate lyase 2 domain-containing protein
Chain: A
Length: 236 amino acids
Theoretical weight: 25.78 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WP3 (Residues: 81-308; Coverage: 74%)
Gene name: aly
Pfam: Alginate lyase
InterPro:
CATH: Jelly Rolls
Alginate lyase 2 domain-containing protein in PDB entry 2zac, assembly 1, front view.
Alginate lyase 2 domain-containing protein in PDB entry 2zac, assembly 1, side view.
Alginate lyase 2 domain-containing protein in PDB entry 2zac, assembly 1, top view.
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