› 2bnx

X-ray diffraction
2.4Å resolution

Crystal structure of the dimeric regulatory domain of mouse diaphaneous-related formin (DRF), mDia1

Released:
DOI: 10.2210/pdb2bnx/pdb
PDB entry 2bnx coloured by chain, front view.
PDB entry 2bnx coloured by chain, side view.
PDB entry 2bnx coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of Rho GTPase-mediated activation of the formin mDia1.
Otomo T, Otomo C, Tomchick DR, Machius M, Rosen MK
Mol Cell 18 273-81 (2005)
PMID: 15866170

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein diaphanous homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 44.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O08808 (Residues: 131-516; Coverage: 31%)
Gene names: Diap1, Diaph1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P32
Unit cell:
a: 121.365Å b: 121.365Å c: 95.096Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.196 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

41 review citations

Regulation of actin filament assembly by Arp2/3 complex and formins.
Pollard TD. (2007)
40 more

9 mentions without citation

Mutations in INF2 are a major cause of autosomal dominant focal segmental glomerulosclerosis.
Boyer et al. (2011)
8 more