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Structure of the fluorescent protein cmFP512 at 1.35A from Cerianthus membranaceus

X-ray diffraction
1.35Å resolution
PDB entry 2c9j coloured by chain, front view.
PDB entry 2c9j coloured by chain, side view.
PDB entry 2c9j coloured by chain, top view.
Source organism: Cerianthus membranaceus
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Green fluorescent protein FP512
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Multimeric state: homo tetramer
Accessible surface area: 29099.04 Å2
Buried surface area: 12213.91 Å2
Dissociation area: 2,803.25 Å2
Dissociation energy (ΔGdiss): 19.44 kcal/mol
Dissociation entropy (TΔSdiss): 14.51 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-178560
Assembly 2
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Assembly Name: Green fluorescent protein FP512
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Multimeric state: homo tetramer
Accessible surface area: 29157.13 Å2
Buried surface area: 11811.48 Å2
Dissociation area: 2,793.96 Å2
Dissociation energy (ΔGdiss): 17.49 kcal/mol
Dissociation entropy (TΔSdiss): 14.51 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-178560

Macromolecules

Green fluorescent protein FP512
Chains: A, B, C, D, E, F, G, H
Length: 223 amino acids
Theoretical weight: 25.33 KDa
Source organism: Cerianthus membranaceus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZQQ5 (Residues: 1-225; Coverage: 100%)
Pfam: Green fluorescent protein
InterPro:
CATH: Green fluorescent protein
Green fluorescent protein FP512 in PDB entry 2c9j, assembly 1, front view.
Green fluorescent protein FP512 in PDB entry 2c9j, assembly 1, side view.
Green fluorescent protein FP512 in PDB entry 2c9j, assembly 1, top view.
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