EC 2.8.1.13: tRNA-uridine 2-sulfurtransferase
Reaction catalysed:
A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor
Systematic name:
[Protein]-S-sulfanyl-L-cysteine:tRNA (uracil(34)-2-O)-sulfurtransferase
Alternative Name(s):
- MnmA (gene name)
GO terms
Biochemical function:
Biological process:
Cellular component:
Sequence families
Protein families (Pfam)
![The deposited structure of PDB entry 2deu contains 2 copies of Pfam domain PF20258 (Aminomethyltransferase beta-barrel domain) in tRNA-specific 2-thiouridylase MnmA. Showing 1 copy in chain C [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2deu_2_A_Pfam_PF20258_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2deu</span> contains 2 copies of Pfam domain <span class='highlight'>PF20258 (Aminomethyltransferase beta-barrel domain)</span> in <span class='highlight'>tRNA-specific 2-thiouridylase MnmA</span>. Showing 1 copy in chain <span class='highlight'>C [auth A]</span>.")
![The deposited structure of PDB entry 2deu contains 2 copies of Pfam domain PF20259 (tRNA methyl transferase PRC-barrel domain) in tRNA-specific 2-thiouridylase MnmA. Showing 1 copy in chain C [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2deu_2_A_Pfam_PF20259_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2deu</span> contains 2 copies of Pfam domain <span class='highlight'>PF20259 (tRNA methyl transferase PRC-barrel domain)</span> in <span class='highlight'>tRNA-specific 2-thiouridylase MnmA</span>. Showing 1 copy in chain <span class='highlight'>C [auth A]</span>.")
![The deposited structure of PDB entry 2deu contains 2 copies of Pfam domain PF03054 (tRNA methyl transferase HUP domain) in tRNA-specific 2-thiouridylase MnmA. Showing 1 copy in chain C [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2deu_2_A_Pfam_PF03054_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2deu</span> contains 2 copies of Pfam domain <span class='highlight'>PF03054 (tRNA methyl transferase HUP domain)</span> in <span class='highlight'>tRNA-specific 2-thiouridylase MnmA</span>. Showing 1 copy in chain <span class='highlight'>C [auth A]</span>.")
RNA family (Rfam)
![The deposited structure of PDB entry 2deu contains 2 copies of Rfam domain RF00005 (tRNA) in tRNA. Showing 1 copy in chain A [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/2deu_1_C_Rfam_RF00005_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2deu</span> contains 2 copies of Rfam domain <span class='highlight'>RF00005 (tRNA)</span> in <span class='highlight'>tRNA</span>. Showing 1 copy in chain <span class='highlight'>A [auth C]</span>.")
InterPro annotations
IPR046885
Domain description:
tRNA-specific 2-thiouridylase MnmA-like, C-terminal domain
Occurring in:
Occurring in:
IPR023382
Domain description:
tRNA-specific 2-thiouridylase MnmA-like, central domain superfamily
Occurring in:
Occurring in:
Structure domains
CATH domains
2.40.30.10
Class:
Mainly Beta
Architecture:
Beta Barrel
Topology:
Elongation Factor Tu (Ef-tu); domain 3
Homology:
Translation factors
Occurring in:
![The deposited structure of PDB entry 2deu contains 2 copies of CATH domain 2.40.30.10 (Elongation Factor Tu (Ef-tu); domain 3) in tRNA-specific 2-thiouridylase MnmA. Showing 1 copy in chain C [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2deu_2_A_CATH_2.40.30.10_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2deu</span> contains 2 copies of CATH domain <span class='highlight'>2.40.30.10 (Elongation Factor Tu (Ef-tu); domain 3)</span> in <span class='highlight'>tRNA-specific 2-thiouridylase MnmA</span>. Showing 1 copy in chain <span class='highlight'>C [auth A]</span>.")
2.30.30.280
Class:
Mainly Beta
Architecture:
Roll
Topology:
SH3 type barrels.
Homology:
Adenine nucleotide alpha hydrolases-like domains
Occurring in:
![The deposited structure of PDB entry 2deu contains 2 copies of CATH domain 2.30.30.280 (SH3 type barrels.) in tRNA-specific 2-thiouridylase MnmA. Showing 1 copy in chain C [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2deu_2_A_CATH_2.30.30.280_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2deu</span> contains 2 copies of CATH domain <span class='highlight'>2.30.30.280 (SH3 type barrels.)</span> in <span class='highlight'>tRNA-specific 2-thiouridylase MnmA</span>. Showing 1 copy in chain <span class='highlight'>C [auth A]</span>.")
![The deposited structure of PDB entry 2deu contains 2 copies of CATH domain 3.40.50.620 (Rossmann fold) in tRNA-specific 2-thiouridylase MnmA. Showing 1 copy in chain C [auth A].](https://www.ebi.ac.uk/pdbe/static/entry/2deu_2_A_CATH_3.40.50.620_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>2deu</span> contains 2 copies of CATH domain <span class='highlight'>3.40.50.620 (Rossmann fold)</span> in <span class='highlight'>tRNA-specific 2-thiouridylase MnmA</span>. Showing 1 copy in chain <span class='highlight'>C [auth A]</span>.")