› 2dfu

X-ray diffraction
2.2Å resolution

Crystal structure of the 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase from Thermus Thermophilus HB8

Released:
DOI: 10.2210/pdb2dfu/pdb
PDB entry 2dfu coloured by chain, front view.
PDB entry 2dfu coloured by chain, side view.
PDB entry 2dfu coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
5-carboxymethyl-2-hydroxymuconate = (3E,5R)-5-carboxy-2-oxohept-3-enedioate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178062 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 264 amino acids
Theoretical weight: 29.42 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SK43 (Residues: 1-264; Coverage: 100%)
Gene name: TTHA0809
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 93.34Å b: 73.412Å c: 122.632Å
α: 90° β: 111.78° γ: 90°
R-values:
R R work R free
0.198 0.197 0.224
Expression system: Escherichia coli BL21(DE3)

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