› 2ees

X-ray diffraction
1.75Å resolution

Guanine riboswitch A21U, U75A mutant bound to hypoxanthine

Released:
DOI: 10.2210/pdb2ees/pdb
PDB entry 2ees coloured by chain, front view.
PDB entry 2ees coloured by chain, side view.
PDB entry 2ees coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Mutational analysis of the purine riboswitch aptamer domain.
Gilbert SD, Love CE, Edwards AL, Batey RT
Biochemistry 46 13297-309 (2007)
PMID: 17960911

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250427 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Guanine riboswitch Chain: A
Molecule details ›
Chain: A
Length: 67 nucleotides
Theoretical weight: 21.51 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
Sequence domains: Purine riboswitch

Ligands and Environments

3 bound ligands:
Ligand ACT 1 x ACT
Ligand NCO 12 x NCO
Ligand HPA 1 x HPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 132.54Å b: 35.96Å c: 41.47Å
α: 90° β: 91.31° γ: 90°
R-values:
R R work R free
0.207 0.207 0.241
Expression system: Not provided

Quick links

Citations

12 review citations

RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.
Šponer et al. (2018)
11 more

11 mentions without citation

Using RNA Sequence and Structure for the Prediction of Riboswitch Aptamer: A Comprehensive Review of Available Software and Tools.
Antunes et al. (2017)
10 more