Structure analysis

Crystal structure of the C-terminal tropomyosin fragment with N- and C-terminal extensions of the leucine zipper at 2.0 angstroms resolution

X-ray diffraction
2Å resolution
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: homo dimer
Accessible surface area: 22280.32 Å2
Buried surface area: 8339.28 Å2
Dissociation area: 4,169.64 Å2
Dissociation energy (ΔGdiss): 79.2 kcal/mol
Dissociation entropy (TΔSdiss): 14.37 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-136513
Assembly 2
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Multimeric state: homo dimer
Accessible surface area: 22385.24 Å2
Buried surface area: 8234.79 Å2
Dissociation area: 4,117.4 Å2
Dissociation energy (ΔGdiss): 81.14 kcal/mol
Dissociation entropy (TΔSdiss): 14.37 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-136513

Macromolecules

Chains: A, B, C, D
Length: 155 amino acids
Theoretical weight: 18.34 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03069 (Residues: 249-277; Coverage: 10%)
  • Canonical: P58772 (Residues: 176-273; Coverage: 35%)
  • nullnull
Gene names: AAS101, AAS3, ARG9, GCN4, TPM1, TPMA, YEL009C
Pfam:
InterPro:
CATH: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

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