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Model for lentivirus capsid core assembly based on crystal dimers of EIAV p26.

Jin Z, Jin L, Peterson DL, Lawson CL
J Mol Biol 286 83-93 (1999)
Cited: 95 times
EuropePMC logo PMID: 9931251

Abstract

Two crystal forms of recombinant p26 capsid protein (CA) from the equine infectious anemia virus (EIAV) have in common an antiparallel four-helix bundle dimer interface between N-terminal domains (NTDs). The dimer interface provides a lenient scaffold to accommodate the wide sequence variation in these helices within lentivirus CA. Pairs of dimers weakly associate to form exact or approximate D2 symmetry tetramers. In one of the two crystal forms, the tetramers are linked via dimerization of C-terminal domains (CTDs). We propose that the observed NTD and CTD homodimer interactions are involved in the assembly of the lentivirus capsid. The NTD homodimer shape readily suggests a model for the mature capsid core, based on hexagonal packing with dimensions and surface topology resembling described EIAV capsid cores. Combining available data for human immunodeficiency virus and EIAV CA, we also propose an assembly pathway for maturation of the lentivirus capsid core following proteolytic cleavage of the gag polyprotein precursor.

Reviews - 2eia mentioned but not cited (1)

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Related citations provided by authors (1)

  1. Cloning, expression, purification, and characterization of the major core protein (p26) from equine infectious anemia virus.. Birkett AJ, Yélamos B, Rodríguez-Crespo I, Gavilanes F, Peterson DL Biochim Biophys Acta 1339 62-72 (1997)

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