› 2fn7

X-ray diffraction
2.3Å resolution

Crystal structure of the lactate dehydrogenase from cryptosporidium parvum complexed with substrate (lactic acid) and cofactor (b-nicotinamide adenine dinucleotide)

Released:
DOI: 10.2210/pdb2fn7/pdb
PDB entry 2fn7 coloured by chain, front view.
PDB entry 2fn7 coloured by chain, side view.
PDB entry 2fn7 coloured by chain, top view.
Source organism: Cryptosporidium parvum
Entry authors: Senkovich OA, Chattopadhyay D

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-176952 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 33.88 KDa
Source organism: Cryptosporidium parvum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5CYZ2 (Residues: 17-337; Coverage: 95%)
Gene name: cgd7_480
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand LAC 1 x LAC
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 95.47Å b: 95.47Å c: 186.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.205 0.246
Expression system: Escherichia coli

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