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Crystal structure of the soluble Aldose sugar dehydrogenase (Asd) from Escherichia coli in the apo-form

X-ray diffraction
1.5Å resolution
PDB entry 2g8s coloured by chain, front view.
PDB entry 2g8s coloured by chain, side view.
PDB entry 2g8s coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Aldose sugar dehydrogenase YliI
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Multimeric state: monomeric
Accessible surface area: 13702.46 Å2
Buried surface area: 3044.87 Å2
Dissociation area: 115.7 Å2
Dissociation energy (ΔGdiss): -3.24 kcal/mol
Dissociation entropy (TΔSdiss): -0.27 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-550513
Assembly 2
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Assembly Name: Aldose sugar dehydrogenase YliI
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Multimeric state: monomeric
Accessible surface area: 13695.25 Å2
Buried surface area: 2638.07 Å2
Dissociation area: 115.39 Å2
Dissociation energy (ΔGdiss): -3.27 kcal/mol
Dissociation entropy (TΔSdiss): -0.28 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-550513

Macromolecules

Aldose sugar dehydrogenase YliI
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P75804 (Residues: 21-370; Coverage: 100%)
Gene names: JW0821, b0837, yliI
Pfam: Glucose / Sorbosone dehydrogenase
InterPro:
CATH: TolB, C-terminal domain
Aldose sugar dehydrogenase YliI in PDB entry 2g8s, assembly 1, front view.
Aldose sugar dehydrogenase YliI in PDB entry 2g8s, assembly 1, side view.
Aldose sugar dehydrogenase YliI in PDB entry 2g8s, assembly 1, top view.
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