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Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA

X-ray diffraction
2.3Å resolution
PDB entry 2h27 coloured by chain, front view.
PDB entry 2h27 coloured by chain, side view.
PDB entry 2h27 coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1
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Assembly Name: ECF RNA polymerase sigma-E factor and DNA
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Multimeric state: hetero trimer
Accessible surface area: 7858.56 Å2
Buried surface area: 2575.2 Å2
Dissociation area: 764 Å2
Dissociation energy (ΔGdiss): 8.72 kcal/mol
Dissociation entropy (TΔSdiss): 10.11 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-115323
Assembly 2 (preferred)
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Assembly Name: ECF RNA polymerase sigma-E factor and DNA
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Multimeric state: hetero trimer
Accessible surface area: 7570.86 Å2
Buried surface area: 2965.59 Å2
Dissociation area: 927.93 Å2
Dissociation energy (ΔGdiss): 23.26 kcal/mol
Dissociation entropy (TΔSdiss): 9.74 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-115323

Macromolecules

ECF RNA polymerase sigma-E factor
Chains: A, D
Length: 73 amino acids
Theoretical weight: 8.44 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGB6 (Residues: 122-191; Coverage: 37%)
Gene names: JW2557, b2573, rpoE, sigE
Pfam: Sigma-70, region 4
InterPro:
CATH: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
SCOP: Sigma4 domain
ECF RNA polymerase sigma-E factor in PDB entry 2h27, assembly 2, front view.
ECF RNA polymerase sigma-E factor in PDB entry 2h27, assembly 2, side view.
ECF RNA polymerase sigma-E factor in PDB entry 2h27, assembly 2, top view.
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DNA chains B, E
Name: 5'-D(*CP*CP*CP*GP*GP*AP*AP*CP*TP*TP*CP*G)-3'
Representative chains: B, E
Source organism: Escherichia coli K-12 [83333]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.62 KDa
5'-D(*CP*CP*CP*GP*GP*AP*AP*CP*TP*TP*CP*G)-3' in PDB entry 2h27, assembly 2, front view.
5'-D(*CP*CP*CP*GP*GP*AP*AP*CP*TP*TP*CP*G)-3' in PDB entry 2h27, assembly 2, side view.
5'-D(*CP*CP*CP*GP*GP*AP*AP*CP*TP*TP*CP*G)-3' in PDB entry 2h27, assembly 2, top view.
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DNA chains C, F
Name: 5'-D(*C*CP*GP*AP*AP*GP*TP*TP*CP*CP*GP*G)-3'
Representative chains: C, F
Source organism: Escherichia coli K-12 [83333]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
5'-D(*C*CP*GP*AP*AP*GP*TP*TP*CP*CP*GP*G)-3' in PDB entry 2h27, assembly 2, front view.
5'-D(*C*CP*GP*AP*AP*GP*TP*TP*CP*CP*GP*G)-3' in PDB entry 2h27, assembly 2, side view.
5'-D(*C*CP*GP*AP*AP*GP*TP*TP*CP*CP*GP*G)-3' in PDB entry 2h27, assembly 2, top view.
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