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An unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductance

X-ray diffraction
2Å resolution
PDB entry 2npk coloured by chain, front view.
PDB entry 2npk coloured by chain, side view.
PDB entry 2npk coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
homo trimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Ammonium transporter AmtB
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Multimeric state: homo trimer
Accessible surface area: 34371.1 Å2
Buried surface area: 13307.51 Å2
Dissociation area: 5,103.5 Å2
Dissociation energy (ΔGdiss): 126.62 kcal/mol
Dissociation entropy (TΔSdiss): 28.97 kcal/mol
Symmetry number: 3
PDBe Complex ID: PDB-CPX-159623
Homo trimeric assembly 1 of PDB entry 2npk coloured by chemically distinct molecules, front view.
Homo trimeric assembly 1 of PDB entry 2npk coloured by chemically distinct molecules, side view.
Homo trimeric assembly 1 of PDB entry 2npk coloured by chemically distinct molecules, top view.
    Assembly 1
Confidence : 98%
No. subunits : 3
Symmetry : C3
More details
3DComplex & QSbio predictionx
No. subunits : 3
Symmetry : C3
Evidence : quaternary structure geometry is similar to that of 3b9y_1 (24% id)

Macromolecules

Ammonium transporter AmtB
Chain: A
Length: 424 amino acids
Theoretical weight: 44.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69681 (Residues: 23-428; Coverage: 100%)
Gene names: JW0441, amtB, b0451, ybaG
Pfam: Ammonium Transporter Family
InterPro:
CATH: Ammonium transporter AmtB like domains
Ammonium transporter AmtB in PDB entry 2npk, assembly 1, front view.
Ammonium transporter AmtB in PDB entry 2npk, assembly 1, side view.
Ammonium transporter AmtB in PDB entry 2npk, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P69681  
142450100150200250300350400
 
100200300400APAVADKADNAFMMICTALVLFMTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINWLMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTLSYIPIAHMVWGGGLLASHGALDFAGGTVVAINAAIAGLVGAYLIGKRVGFGKEAFKPHNLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRGKPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPCDVFGVAGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVAFIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNADQAQQPAQADLEHHHHHH
UniProt
P69681
Chains
Domains
Secondary structure
Flexibility predictions
Early folding residue predictions
Ligand binding sites
Interaction interfaces
Sequence conservation
Molecule details ›

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