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Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex

X-ray diffraction
2.8Å resolution
PDB entry 2olv coloured by chain, front view.
PDB entry 2olv coloured by chain, side view.
PDB entry 2olv coloured by chain, top view.
Source organism: Staphylococcus aureus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: peptidoglycan glycosyltransferase
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Multimeric state: monomeric
Accessible surface area: 28612.18 Å2
Buried surface area: 1355.47 Å2
Dissociation area: 677.74 Å2
Dissociation energy (ΔGdiss): -8.32 kcal/mol
Dissociation entropy (TΔSdiss): 7.59 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-193115
Assembly 2
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Assembly Name: peptidoglycan glycosyltransferase
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Multimeric state: monomeric
Accessible surface area: 28626.61 Å2
Buried surface area: 1323.64 Å2
Dissociation area: 661.82 Å2
Dissociation energy (ΔGdiss): -6.88 kcal/mol
Dissociation entropy (TΔSdiss): 7.6 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-193115

Macromolecules

peptidoglycan glycosyltransferase
Chains: A, B
Length: 669 amino acids
Theoretical weight: 74.64 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9R744 (Residues: 60-727; Coverage: 92%)
Gene names: ACR74_01585, CNH36_07780, EIG96_05435, EP54_02360, EQ90_10185, GO814_11815, GO942_13395, HMPREF3211_02644, LB359_08280, M1K003_0294, NCTC10702_02314, SAMEA70153168_00413, pbp2, ponA
Pfam:
InterPro:
CATH:
SCOP:
peptidoglycan glycosyltransferase in PDB entry 2olv, assembly 1, front view.
peptidoglycan glycosyltransferase in PDB entry 2olv, assembly 1, side view.
peptidoglycan glycosyltransferase in PDB entry 2olv, assembly 1, top view.
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